Adenovirus-mediated expression of human sodium-iodide symporter gene permits in vivo tracking of adipose tissue-derived stem cells in a canine myocardial infarction model.
Introduction: In vivo tracking of the transplanted stemcells is important in pre-clinical research of stemcell therapy for myocardial infarction. We examined the feasibility of adenovirus-mediated sodium iodide symporter
(NIS) gene to cell tracking imaging of transplanted stem cells in a canine infarctedmyocardiumby clinical single photon emission computed tomography (SPECT).
Methods: Beagle dogs were injected intramyocardially with NIS-expressing adenovirus-transfected canine stem cells (Ad-hNIS-canine ADSCs) a week after myocardial infarction (MI) development. 99mTc- methoxyisobutylisonitrile (99mTc-MIBI) and 99mTc-pertechnetate (99mTcO4−) SPECT imaging were performed for assessment of infarcted myocardium and viable stemcell tracking. Transthoracic chocardiographywas performed tomonitor any functional cardiac changes.
Results: Left ventricular ejection fraction (LVEF) was decreased after LAD ligation. There was no significant difference in EF between the groups with the stemcell or saline injection. 125I uptakewas higher in Ad-hNIS-canine ADSCs than in non-transfected ADSCs. Cell proliferation and differentiation were not affected by hNIS-carrying adenovirus transfection. 99mTc-MIBI myocardial SPECT imaging showed decreased radiotracer uptake in the infarcted apex and midanterolateral regions. Ad-hNIS-canine ADSCs were identified as a region of focally increased 99mTcO4 − uptake at the
lateral wall and around the apex of the left ventricle, peaked at 2 days and was observed until day 9.
Conclusions: Combination of adenovirus-mediated NIS gene transfection and clinical nuclear imaging modalities enables to trace the fate of transplanted stem cells in infarcted myocardium for translational in vivo cell tracking study for prolonged duration
발행연도 : 2015
저자 : Lee AR, Woo SK, Kang SK, Lee SY, Lee MY, Park NW, Song SH, Lee SY, Nahm SS, Yu JE, Kim MH, Yoo RJ, Kang JH, Lee YJ, Eom KD
출처 : Nucl Med Biol
소스 : 지방MSC