Adenovirus-mediated expression of human sodium-iodide symporter gene permits in vivo tracking of adipose tissue-derived stem cells in a canine myocardial infarction model.
Introduction: In vivo tracking of the transplanted stemcells is important in pre-clinical research of stemcell therapy for myocardial infarction. We examined the feasibility of adenovirus-mediated sodium iodide symporter
(NIS) gene to cell tracking imaging of transplanted stem cells in a canine infarctedmyocardiumby clinical single photon emission computed tomography (SPECT).
Methods: Beagle dogs were injected intramyocardially with NIS-expressing adenovirus-transfected canine stem cells (Ad-hNIS-canine ADSCs) a week after myocardial infarction (MI) development. 99mTc- methoxyisobutylisonitrile (99mTc-MIBI) and 99mTc-pertechnetate (99mTcO4−) SPECT imaging were performed for assessment of infarcted myocardium and viable stemcell tracking. Transthoracic chocardiographywas performed tomonitor any functional cardiac changes.